EC Number   |
Protein Variants |
Reference |
|---|
    1.1.1.203 | A172V |
the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme |
762310 |
| 1.1.1.203 | A39P |
the mutant shows 103.5% activity compared to the wild type enzyme |
-, 760723 |
| 1.1.1.203 | A39P/H234K |
the mutant shows 165.7% activity compared to the wild type enzyme |
760723 |
| 1.1.1.203 | A39P/H99Y |
the mutant shows 146.8% activity compared to the wild type enzyme |
-, 760723 |
| 1.1.1.203 | A39P/H99Y/H234K |
the triple mutant shows 247.6% activity compared to the wild type enzyme, a 400fold improvement in half-life at 59°C, and a 5°C improvement in T50 (10 min) value |
-, 760723 |
| 1.1.1.203 | A41P/H101N/H236K |
the mutant shows 52.3% catalytic efficiency as compared to the wild type enzyme |
760328 |
| 1.1.1.203 | A41P/H101N/H236R |
the mutant shows 59.3% catalytic efficiency as compared to the wild type enzyme |
760328 |
| 1.1.1.203 | A41P/H101Y/H236K |
the mutant shows 58.4% catalytic efficiency, higher kinetic and thermodynamic stability with a T50 value of 62.2°C after 15 min (3.2°C improvement) as compared to the wild type enzyme |
760328 |
| 1.1.1.203 | A41P/H101Y/H236R |
the mutant shows 56% catalytic efficiency as compared to the wild type enzyme |
760328 |
| 1.1.1.203 | A41P/H236K |
the mutant shows 62.7% catalytic efficiency as compared to the wild type enzyme |
760328 |
