EC Number   |
Protein Variants |
Reference |
|---|
   1.1.1.12 | D211S |
strong decrease in activity |
712767 |
| 1.1.1.12 | D211S/I212R |
strong decrease in activity, increase in activity with cofactor NADP+ |
712767 |
| 1.1.1.12 | D211S/I212R/D213N |
strong decrease in activity, increase in activity with cofactor NADP+ |
712767 |
| 1.1.1.12 | D211S/I212R/S348T |
strong decrease in activity, increase in activity with cofactor NADP+ |
712767 |
| 1.1.1.12 | D212S/I213R |
no activity with NAD+, slight activity with NADP+ |
-, 710970 |
| 1.1.1.12 | D213S/I214R |
significantly higher preference for NADP+ over NAD+ |
710970 |
| 1.1.1.12 | D224S/I225R |
significantly altered cofactor specificity from NAD+ to NADP+ |
710970 |
| 1.1.1.12 | H91A |
site-directed mutagenesis, catalytically inactive mutant |
-, 740145 |
| 1.1.1.12 | M70F |
almost complete loss of activity |
696988 |
| 1.1.1.12 | M70F |
results in a nearly complete enzyme inactivation |
696988 |
