EC Number   |
Protein Variants |
Reference |
|---|
   1.1.1.10 | D207/I208R/F209S |
kcat/Km of mutant enzyme for NAD+ dropps 15fold compared with the native enzyme, kcat/Km for NADP+ increases up to 4100fold |
688749 |
| 1.1.1.10 | D238E |
site-directed mutagenesis, mutant exists in dimeric form at low temperature like the wild-type enzyme resulting in cold inactivation |
654438 |
| 1.1.1.10 | D238E/L242W |
site-directed mutagenesis, mutation leads to complete prevention of cold inactivation, mutant exists in tetrameric form at low temperature |
654438 |
| 1.1.1.10 | D238E/L242W/T244C |
site-directed mutagenesis, double mutation leads to partial prevention of cold inactivation, mutant exists in dimeric and tetrameric form at low temperature |
654438 |
| 1.1.1.10 | H146L |
IC50-value for 4-methyl-[1,2,3]-thiadiazole-5-carboxylic acid benzyloxyamide is 7.8fold higher than wild-type value, IC50-value for 4-methylthiophene-2-carboxylic acid N'-(2,3,3-trichloroacryloyl)-hydrazide is 6.7fold higher than wild-type value |
670839 |
| 1.1.1.10 | H146L |
site-directed mutagenesis, altered activity |
655222 |
| 1.1.1.10 | K153M |
site-directed mutagenesis, active site mutant, complete loss of activity |
655222 |
| 1.1.1.10 | L143F |
IC50-value for 4-methyl-[1,2,3]-thiadiazole-5-carboxylic acid benzyloxyamide is 8.3fold higher than wild-type value, IC50-value for 4-methylthiophene-2-carboxylic acid N'-(2,3,3-trichloroacryloyl)-hydrazide is 1.9fold higher than wild-type value |
670839 |
| 1.1.1.10 | L143F |
site-directed mutagenesis, altered activity |
655222 |
| 1.1.1.10 | L242W |
site-directed mutagenesis, mutation leads to partial prevention of cold inactivation, mutant exists in dimeric and tetrameric form at low temperature |
654438 |
