EC Number   |
Protein Variants |
Reference  |
|---|
    5.1.1.5 | T224I |
site-directed mutagenesis, the mutant shows significantly decreased activity compared to the wild-type enzyme |
727795 |
| 5.1.1.5 | T224I/W355Y |
site-directed mutagenesis, the double mutant shows significantly decreased activity compared to the wild-type enzyme |
727795 |
| 5.1.1.5 | W355Y |
site-directed mutagenesis, the mutant shows significantly decreased activity compared to the wild-type enzyme |
727795 |
| 5.1.1.5 | A165K/N174L/T391Y |
site-directed mutagenesis, the mutant shows no activity towards L-Ala, L-Lys, or L-Arg |
-, 728587 |
| 5.1.1.5 | N174L |
site-directed mutagenesis, inactive mutant |
-, 728587 |
| 5.1.1.5 | R173A |
site-directed mutagenesis, inactive mutant |
-, 728587 |
| 5.1.1.5 | R173K |
site-directed mutagenesis, inactive mutant |
-, 728587 |
| 5.1.1.5 | T391Y |
site-directed mutagenesis, the mutant shows 47% reduced activity with L-lysine compared to the wild-type enzyme |
-, 728587 |
| 5.1.1.5 | T391Y/S394Y |
site-directed mutagenesis,the mutant exhibits significantly reduced specific activity towards L-Lys (6% residual activity) and toward L-Arg (0.9% residual activity) |
728587 |
| 5.1.1.5 | S394C |
site-directed mutagenesis, the mutant shows 1.5fold increased activity with L-arginine compared to the wild-type enzyme |
-, 728711 |
