EC Number   |
Protein Variants |
Reference |
|---|
  4.99.1.9 | E264Q |
21% of wild-type activity |
678312 |
| 4.99.1.9 | E264V |
less than 1% of wild-type activity |
678312 |
| 4.99.1.9 | H183A |
less than 1% of wild-type activity |
678312 |
| 4.99.1.9 | H183C |
less than 1% of wild-type activity |
678312 |
| 4.99.1.9 | H88A |
5% of wild-type activity |
678312 |
| 4.99.1.9 | K87A |
92% of wild-type activity |
678312 |
| 4.99.1.9 | more |
all the non-wild-type LmCpfC variants prove to have a significantly higher koff and consequently a lower affinity. This weaker binding is also reflected in the kon-rate, which is the highest for both tested porphyrin ligands for the wild-type protein |
771075 |
| 4.99.1.9 | R29L |
site-directed mutagenesis, the mutant shows altered ligand binding, kinetics, and thermostability compared to wild-type enzyme |
771075 |
| 4.99.1.9 | R45L |
site-directed mutagenesis, the mutant lacks the H-bonding with the propionate at position 6 (p6), determination and analysis of the mutant enzyme structure as apo-enzyme and with bound substrate coproporphyrin III, structure comparison with the wild-type enzyme, overview |
775669 |
| 4.99.1.9 | R45L |
site-directed mutagenesis, the mutant shows altered ligand binding, kinetics, and thermostability compared to wild-type enzyme |
771075 |
