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Results 1 - 4 of 4
EC Number
Amino acid exchange
Commentary
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alpha-humulene production using the engineered Escherichia coli BL21(DE3) strain in a 2-l bioreactor cultivation, method optimization, overview. Coordinating Cu2+ ions to the NTA ligands in affinity chromatography results in a high non-specific binding of endogenous host cell proteins due to the high affinity of Cu2+ to histidine groups. Zn2+-IMAC shows much less impurities in eluted protein fractions which is consistent with the fact that Zn2+ ions are less affine to histidine groups. The recovery rate of 80 % of the protein of interest using Zn2+ ions is the lowest compared to all other metal ions tested. Ni2+- and Co2+-IMAC seem to be similar in product purity (60% and 63%) and recovery (93% and 95%). As Co2+-IMAC shows the best purity to recovery ratio, further purification is done using Co2+-IMAC
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de novo production of zerumbone is achieved in a metabolically engineered yeast cell factory by introducing alpha-humulene synthase (ZSS1), alpha-humulene 8-hydroxylase (CYP71BA1) and zerumbone synthase variant (ZSD1S114A) from Zingiber zerumbet, together with AtCPR1 from Arabidopsis thaliana into the Saccharomyces cerevisiae strain. Multistep metabolic engineering strategies are applied, including the overexpression of the mevalonate (MVA) pathway rate-limiting enzymes tHMG1 and yeast ERG20, regulation of yeast ERG9 by an inducible promoter and competitive pathway deletion to redirect metabolic flux toward the desired product, method evaluation and optimization, overview. In the engineered strain, alpha-humulene production increases by 18fold, to 92 mg/l compared to that in the original strain, fed-batch fermentation using Saccharomyces cerevisiae strain LW16 in a 5-l bioreactor
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heterologous expression of the mevalonate pathway and alpha-humulene synthase results in the production of alpha-humulene from CO2 under heterotrophic conditions. The production system is used to produce 17 mg alpha-humulene per gram cell dry mass (CDW) from CO2 and electrical energy in microbial electrosynthesis (MES) mode
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potential of Methylobacterium extorquens as a future platform host for the production of high-value terpenoids from the alternative carbon source methanol. Inducible recombinant coexpression of alpha-humulene synthasefrom Zingiber zerumbet with farnesyl diphosphate (FPP) synthase from Saccharomyces cerevisiae leads to concentrations of up to 18 mg/l alpha-humulene in Methylobacterium extorquens strain AM1. Extracellular alpha-humulene has no growth inhibiting effect on Methylobacterium extorquens strain AM1 up to concentrations of 1 g/l. Method evaluation, overview
Results 1 - 4 of 4