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Results 1 - 5 of 5
EC Number
Protein Variants
Commentary
Reference
R265H
no AdoMet synthetase activity, normal tripolyphosphatase activity
R265S
no AdoMet synthetase activity, reduced tripolyphosphatase activity
K52R
the His-tagged mutant loses 90-99% of its activity (catalytic efficiency is at least 1000times lower) compared to the wild type enzyme. Mn2+ does not induce a significant activation of this mutant
K85A
the His-tagged mutant is about 10times less active than the wild type enzyme, but the optimal conditions for activity are essentially the same. The mutant is more strongly activated by Mn2+ than by Mg2+, and the inhibitory effects of Ca2+ and Zn2+ are less pronounced
K8A
the His-tagged mutant remains highly active with tripolyphosphate as substrate and Mg2+ as activator, with a catalytic efficiency close to that of the recombinant wild type enzyme
Results 1 - 5 of 5