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Results 1 - 10 of 10
EC Number
Protein Variants
Commentary
Reference
E138A
no enzymic activity, no change in overall structure compared to wild-type
E138A
site-directed mutagenesis, inactive mutant, dTTP is bound to the active site of E138A, the region between Val120 and His125 is formed in a new conformation, the C-terminal fold is disordered
E138D
site-directed mutagenesis, the mutant enzyme shows a 140fold reduction in kcat and altered dTTP inhibition compared to the wild-type enzyme
E138Q
no enzymic activity, no change in overall structure compared to wild-type
H121A
site-directed mutagenesis, inactive mutant, dTTP is bound to the active site of E138A, the region between Val120 and His125 is formed in a new conformation, the C-terminal fold is disordered
R115A
no enzymic activity, no change in overall structure compared to wild-type
R115Q
site-directed mutagenesis, inactive mutant
S111C
site-directed mutagenesis, inactive mutant
S111T
site-directed mutagenesis, the mutant enzyme shows a 30fold reduction in kcat and altered dTTP inhibition compared to the wild-type enzyme, modeling of the active site of the S111T enzyme, overview
V122G
site-directed mutagenesis, inactive mutant
Results 1 - 10 of 10