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Results 1 - 10 of 13 > >>
EC Number
Amino acid exchange
Commentary
Reference
C438S
site-directed mutagenesis
C462A
recombinant protein
C462S
does not restore the ability of Salmonella to eliminate phosphatidylinositol 4,5-bisphosphate in SigD-deficient bacteria
C462S
no functional or morphological changes in epithelial cells transfected with phosphatase-deficient mutant C462S
DELTA1-135-DELTA118-142
recombinant protein, Saccharomyces cerevisiae: no effect in Saccharomyces cerevisiae, HeLa: no effect (Green Fluorescent Protein linked) in HeLa
DELTA1-165
recombinant protein, Saccharomyces cerevisiae: strong impact on actin filaments and budding, HeLa (Green Fluorescent Protein linked): loss of adherence and strong impact on actin filaments
DELTA1-351
recombinant protein, Saccharomyces cerevisiae: strong impact on actin filaments and budding, HeLa (Green Fluorescent Protein linked): loss of adherence and strong impact on actin filaments
DELTA118-142
recombinant protein, Saccharomyces cerevisiae: strong impact on actin filaments and budding, residues 118–142 crucial for toxicity of SigD in the yeast cells, affecting both phosphatase activity and actin depolarization events, no effect (Green Fluorescent Protein linked) in HeLa
DELTA352-563
recombinant protein, no effect in Saccharomyces cerevisiae, no effect in HeLa (Green Fluorescent Protein linked)
K527A
restores the ability of Salmonella to eliminate phosphatidylinositol 4,5-bisphosphate in SigD-deficient bacteria
Results 1 - 10 of 13 > >>