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EC Number	Protein Variants	Commentary	Reference
2.9.1.2	H166A	the mutant is partially active in forming Sec-tRNASec in vivo. In vitro, the mutant is partially active in forming Cys-tRNASec	694428
2.9.1.2	H166F	mutant is inactive in vivo	694428
2.9.1.2	H166Q	mutant is inactive in vivo	694428
2.9.1.2	R307A	the mutant is significantly less active in L-selenocysteinyl-tRNASec formation in vivo and Cys-tRNASec formation in vitro	694428
2.9.1.2	R72A	the mutant enzyme is significantly less active in L-selenocysteinyl-tRNASec formation in vivo and Cys-tRNASec formation in vitro. The mutant enzyme is unable to form L-selenocysteinyl-tRNASec in vitro	694428
2.9.1.2	K173A	in vivo activity of the mutant is indistinguishable from that of the wild-type enzyme	695136
2.9.1.2	K173M	in vivo activity of the mutant is indistinguishable from that of the wild-type enzyme	695136
2.9.1.2	Q105A	mutant is inactive in vivo	695136
2.9.1.2	R313A	mutant is inactive in vivo	695136
2.9.1.2	R75A	mutant is inactive in vivo	695136

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Release 2023.1 (January 2023)