EC Number   |
Protein Variants |
Reference |
|---|
    2.7.6.2 | more |
construction of a double knockout mutant lacking isozymes TPK1 and TPK2, the mutant shows severely depressed levels of TPP and elevated levels of free thiamine compared to the wild-type enzyme |
676560 |
| 2.7.6.2 | S74A |
mutation causes a 1.4fold increase in turnover number, the Km-value for ATP is 2fold that of the wild-type enzyme |
642743 |
| 2.7.6.2 | D133N |
mutation causes a selective decrease in the ratio of turnover-number to Km-value for thiamine |
642743 |
| 2.7.6.2 | Q96E |
mutation causes an 2.5fold increase in the ratio of turnover-number to Km-value for thiamine compared to the wild-type |
642743 |
| 2.7.6.2 | R131G |
mutation decreases the ratio of turnover-number to Km-value for ATP |
642743 |
| 2.7.6.2 | T99A |
mutation decreases the ratio of turnover-number to Km-value for ATP |
642743 |
| 2.7.6.2 | D100N |
mutation reduces turnover-number markedly |
642743 |
| 2.7.6.2 | D71N |
mutation reduces turnover-number markedly |
642743 |
| 2.7.6.2 | D73N |
mutation reduces turnover-number markedly |
642743 |
| 2.7.6.2 | D222H |
naturally occurring homozygous TPK1 mutation in a patient with enzyme deficiency suffering neurological disorder |
739080 |
