EC Number   |
Protein Variants  |
Reference |
|---|
    2.7.4.8 | C3S/C5S |
PSD-95aC3S/C5S mutant |
700475 |
| 2.7.4.8 | D103N |
active |
642706 |
| 2.7.4.8 | D74A |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
700079 |
| 2.7.4.8 | E101D |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
700079 |
| 2.7.4.8 | E72Q |
no guanylate or adenylate kinase activity |
642706 |
| 2.7.4.8 | E72Q/D103D |
no active |
642706 |
| 2.7.4.8 | G3A |
decrease in Tm-value by 1.4°C as compared to wild-type enzyme. Decrease in catalytic efficiency (kcat/Km) by 14% |
761496 |
| 2.7.4.8 | more |
construction of mutant PSD-95 SH3-GK which contains a deleterious point mutation in the C-terminal SH3 domain |
676138 |
| 2.7.4.8 | more |
RNAi knockdown of the cytosolic enzyme, a v2 mutant is temperature-sensitive and develops chlorotic leaves at restrictive temperatures, the v2 mutation causes inhibition of chloroplast differentiation; in particular, it disrupts the chloroplast translation machinery during early leaf development |
700744 |
| 2.7.4.8 | N103D |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
700079 |
