EC Number   |
Protein Variants |
Reference  |
|---|
    2.4.2.17 | E130A |
site-directed mutagenesis, about 60% reduced activity compared to the wild-type enzyme, no inhibition by histidine |
659495 |
| 2.4.2.17 | Y268F/Y269F |
site-directed mutagenesis, about 30% reduced activity compared to the wild-type enzyme, no inhibition by histidine |
659495 |
| 2.4.2.17 | D155A |
slight increase in kcat, 3.9fold increase in KM-value for 5-phospho-alpha-D-ribose 1-diphosphate, 1.7fold increase in Km-value for ATP |
678174 |
| 2.4.2.17 | K50A |
2.3fold increase in kcat, 51fold increase in KM-value for 5-phospho-alpha-D-ribose 1-diphosphate, 1.8fold increase in Km-value for ATP |
678174 |
| 2.4.2.17 | K8A |
2.5fold decrease in kcat, 4.9fold increase in KM-value for 5-phospho-alpha-D-ribose 1-diphosphate, 3.1fold increase in Km-value for ATP |
678174 |
| 2.4.2.17 | S140A |
mutant is unstable, kinetic parameters can not be determined |
678174 |
| 2.4.2.17 | T159A |
2.2fold decrease in kcat, 280fold increase in KM-value for 5-phospho-alpha-D-ribose 1-diphosphate, 2.5fold decrease in Km-value for ATP |
678174 |
| 2.4.2.17 | T162A |
3.2fold decrease in kcat, 49fold increase in KM-value for 5-phospho-alpha-D-ribose 1-diphosphate, 2.6fold decrease in Km-value for ATP |
678174 |
| 2.4.2.17 | A270P |
conserved residue |
721765 |
| 2.4.2.17 | L231F/T235A |
conserved residues |
721765 |
