EC Number |
Protein Variants |
Reference |
---|
2.1.1.224 | more |
construction of chimera between Cfr and RlmN from Echerichia coli to analyze C2/C8 and C2 methylation specificity, respectively. The catalytic site is expected to be responsible for the C2/C8 specificity. Almost all replacements show no function in the primer extension assay, apart from a few that have a weak effect |
758128 |
2.1.1.224 | more |
construction of chimeric mutants of gene cfr, cfr1234567rrlmN and rlmN1234567rcfr, and construction of mixed genes containing gene fragment of cfr and rlmN genes, overview. Analysis of effects of the mutations on catalytic activity and antibiotics resistance |
758128 |
2.1.1.224 | C112A |
inactive mutant enzyme |
704203 |
2.1.1.224 | C116A |
inactive mutant enzyme |
704203 |
2.1.1.224 | C119A |
inactive mutant enzyme |
704203 |
2.1.1.224 | more |
insertion of the cfr region into the CM05 chromosome occurs at a beta/six site I recognition site. Identification of a subpopulation of cfr-negative CM05, CM05DELTA, arising from recombination between duplicate ermB regions, overview |
-, 718598 |
2.1.1.224 | H214A |
moderately decreased resistance to both florfenicol and tiamulin |
705983 |
2.1.1.224 | C110A |
mutation does not affect Cfr activity |
705983 |
2.1.1.224 | C105A |
mutation eliminates the resistance to both florfenicol and tiamulin |
705983 |
2.1.1.224 | C112A |
mutation eliminates the resistance to both florfenicol and tiamulin |
705983 |