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Results 1 - 7 of 7
EC Number
Amino acid exchange
Commentary
Reference
E291L
sixfold faster turnover than wild-type. Crystallization in same space group as wild-type, but with strikingly different cell constants. Appears as monomer in the crystal
F74Y
OPR3 mutant, change in substrate specificity, similar increase in stereoselectivity is observed for the mutant as compared to the wild-type enzyme
F74Y/H244Y
OPR3 double-mutant, reduction of (9S,13S)-12-oxo-phytodienoic acid is slower in the double-mutant as in the wild type enzyme and the single mutants, the protein crystallizes as a monomer with none of the dimer interactions retain
H244Y
OPR3 mutant, change in substrate specificity, similar increase in stereoselectivity is observed for the mutant as compared to the wild-type enzyme
more
construction of isozyme OPR3 mutant plants for subcellular localization study of the isozyme, overview
more
overexpression of TaOPR1 in wheat significantly enhances the level of salinity tolerance, while its heterologous expression in Arabidopsis thaliana alleviates root growth restriction in the presence of salinity and oxidants and raises the sensitivity to abscisic acid. In Arabidopsis, recombinant TaOPR1 promotes abscisic acid synthesis. and the abscisic acid-dependent stress-responsive pathway. Recombinant TaOPR1 partially rescues the sensitivity of the Arabidopsis thaliana aba2 mutant defective in abscisic acid synthesis to salinity, and improves the activities of reactive oxygen species scavengers and the transcription of their encoding genes while reducing malondialdehyde and reactive oxygen species levels. After exposure to 200 mM NaCl, the wild-type seedlings become very wilted while the TaOE ones remain fully turgid. The TaOE line shoots and roots are longer than those of the wild type, phenotype, overview. Inconsistent with the positive effect of TaOPR1, the double RNA interference (RNAi) knockdown mutant opr1opr2, from two homologous OPRI genes in Arabidopsis thaliana, do not increase the sensitivity to NaCl treatment, and their phenotypes are comparable to the wild-type. TaOPR1-overexpressing lines show insensitivity to H2O2
Y364F
crystallization as a monomer with none of the dimer interactions retained
Results 1 - 7 of 7