EC Number   |
Protein Variants |
Reference |
|---|
   1.3.1.20 | C217A |
4fold increase in Km for 5alpha-androstan-3,17-dione and 2fold increase in Km for NADH |
349257 |
| 1.3.1.20 | R148A |
activity and temperature stability are similar to the wild type enzyme |
689972 |
| 1.3.1.20 | R202A |
activity and temperature stability are similar to the wild type enzyme |
689972 |
| 1.3.1.20 | more |
CDD1-4 mutant consisting of DD1 with C-terminal end of DD4 shows 10-37fold increase in Km for indan-1-ol and 1,2,3,4-tetrahydronaphth-1-ol and significantly decreased activity towards androstanes, higher activity with bile-acids and 5beta-steroid than DD1 |
349261 |
| 1.3.1.20 | more |
CDD4-1 mutant consisting of DD4 with C-terminal end of DD1shows new stereospecificity toward indan-1-ol stereomers and increased Km-values with bile acids and 3-hydroxysteroids compared to DD4 |
349261 |
| 1.3.1.20 | more |
constitutive overexpression of enzyme in 2008 cells leads to induction of cisplatin resistance. In cisplatin-resistant cell-line 2008/C13*, differential expression of enzyme and four more genes is found |
656043 |
| 1.3.1.20 | S145C/L311V |
constructed double mutant with 3 to 5fold decreased activity for xenobiotic and steroidal substrates |
349252 |
| 1.3.1.20 | C87S |
DD1 mutant |
349261 |
| 1.3.1.20 | Q172L |
DD1 mutant |
349261 |
| 1.3.1.20 | R170H |
DD1 mutant |
349261 |
