EC Number |
Protein Variants |
Reference |
---|
1.14.19.9 | F103A |
the mutant shows increased Km and reduced kcat values compared to the wild type enzyme |
726682 |
1.14.19.9 | F103A/W455A |
inactive |
726682 |
1.14.19.9 | H101A |
the mutant shows increased Km and reduced kcat values compared to the wild type enzyme |
726682 |
1.14.19.9 | K79A |
inactive mutant with abolished FAD binding |
685105 |
1.14.19.9 | K79M |
inactive mutant with abolished FAD binding |
685105 |
1.14.19.9 | more |
for optimization of the enzymatic regioselective halogenation process, a method that is easily scalable up to gram scale is established. An active FAD-dependent halogenase is co-immobilized with all auxiliary enzymes necessary for halogenation, formation of cross-linked enzyme aggregates (CLEAs) might stabilize its quaternary structure |
744027 |
1.14.19.9 | more |
gene disruption mutant, no production of pyrroindomycin B, but still production of pyrroindomycin A |
-, 676785 |
1.14.19.9 | W455A |
the mutant shows increased Km and reduced kcat values compared to the wild type enzyme |
726682 |
1.14.19.9 | Y455W |
the mutant preferentially uses tryptamine as substrate |
727668 |