EC Number |
Protein Variants |
Reference |
---|
1.11.1.5 | H71G/W94A |
4% activity compared to the wild type enzyme, contains a high-spin, presumably five-coordinate, peroxidatic heme site |
679215 |
1.11.1.5 | H71G |
55% activity compared to the wild type enzyme, contains a high-spin, presumably five-coordinate, peroxidatic heme site |
679215 |
1.11.1.5 | M118L |
7.3% of wild-type activity |
674744 |
1.11.1.5 | more |
a mutant lacking the putative cytochrome c peroxidase DocA shows a 10fold reduction in colonization of the chick cecum compared to wild-type enzyme, a mutant lacking the putative cytochrome c peroxidase CJJ0382 demonstrates a maximal 50fold colonization defect that is dependent on the inoculum dose |
-, 687074 |
1.11.1.5 | H71G/W94A |
about 4% of wild-type activity. Five-coordinate, peroxidatic heme structure contrary to six-coordinate structure of wild-type, formation of a porphyrin radical species during catalysis |
679215 |
1.11.1.5 | H71G |
about 55% of wild-type activity. Five-coordinate, peroxidatic heme structure contrary to six-coordinate structure of wild-type, formation of a tryptophan radical species during catalysis |
679215 |
1.11.1.5 | W51H |
altered electronic absorption spectra, indicating that the heme group in the mutants is six-coordinate rather than five-coordinate as it is in wild-type cytochrome c peroxidase, weaker effect on cyanide binding, with the cyanide affinity only 2-8times weaker than for cytochrome c peroxidase |
702313 |
1.11.1.5 | W51H/H52L |
altered electronic absorption spectra, indicating that the heme group in the mutants is six-coordinate rather than five-coordinate as it is in wild-type cytochrome c peroxidase, weaker effect on cyanide binding, with the cyanide affinity only 2-8times weaker than for cytochrome c peroxidase |
702313 |
1.11.1.5 | W51H/H52W |
altered electronic absorption spectra, indicating that the heme group in the mutants is six-coordinate rather than five-coordinate as it is in wild-type cytochrome c peroxidase, weaker effect on cyanide binding, with the cyanide affinity only 2-8times weaker than for cytochrome c peroxidase |
702313 |
1.11.1.5 | more |
an unactivated mutant devoid of the protein loop shows 10% of turnover activity of the wild type enzyme in the activated form |
688187 |