EC Number |
Protein Variants |
Reference |
---|
2.1.1.224 | C119A |
mutation of the cysteines in the presumed radical S-adenosyl-L-methionine motif CxxxCxxC abolishes Cfr activity |
706726 |
2.1.1.224 | C119A |
site-directed mutagenesis, the mutant Cfr is inactive, and mutant cells show no resistance against antibiotics |
-, 718587 |
2.1.1.224 | C122A |
mutation of the cysteines in the presumed radical S-adenosyl-L-methionine motif CxxxCxxC abolishes Cfr activity |
706726 |
2.1.1.224 | C338A |
mutation eliminates the resistance to both florfenicol and tiamulin |
705983 |
2.1.1.224 | C338A |
site-directed mutagenesis, Cys338Ala Cfr binds S-adenosyl-L-methionine with equivalent affinity, oxidation of the [4Fe-4S] cluster is not observed |
735095 |
2.1.1.224 | C338A |
the mutant binds S-adenosyl-L-methionine with wild type affinity, while oxidation of the [4Fe-4S] cluster is not observed |
735095 |
2.1.1.224 | E91A |
mutation eliminates the resistance to both florfenicol and tiamulin |
705983 |
2.1.1.224 | F118A |
mutation eliminates the resistance to both florfenicol and tiamulin |
705983 |
2.1.1.224 | H214A |
moderately decreased resistance to both florfenicol and tiamulin |
705983 |
2.1.1.224 | more |
construction of chimera between Cfr and RlmN from Echerichia coli to analyze C2/C8 and C2 methylation specificity, respectively. The catalytic site is expected to be responsible for the C2/C8 specificity. Almost all replacements show no function in the primer extension assay, apart from a few that have a weak effect |
758128 |