EC Number   |
Protein Variants |
Reference |
|---|
   6.2.1.45 | more |
isolation of a mutant in ubiquitin-activiting enzyme Uba1. The mutation alters sensitivity to various environmental stresses and reduces wild-type Uba1 protein function. Protein modification by ubiquitin is strongly impaired in the mutant, inhibiting degradation of ubiquitin-proteasome pathway substrates as well as ubiquitin-dependent but proteasome-independent degradation of membrane receptors |
727501 |
| 6.2.1.45 | more |
MCF-10A cells stably expressing anti-UBA6 shRNA form similar structures as the wild-type cells, but also develop a number (about 5%) of tumor-like gigantic aggregates, approximately 30% of acini formed in the shUBA6 culture do not exhibit hollow lumen. UBA6 knockout cell phenotype, overview |
745952 |
| 6.2.1.45 | more |
mutagenesis of key residues of E1 reveals that its conserved catalytic cysteine residue is essential for the formation of these poyubiquitin chains. Inactivation of the ubiquitin-conjugating enzyme E2 has no effect on the ability of E1 to catalyze ubiquitin chain formation, suggesting E1 is not only responsible for the activaton of ubiquitin but also for the direct extension of the lysine 48-linked polyubiquitin chain by the direct transfer of the ubiquitin-thiolester from the active site of E1 to the terminal Lys48 of the growing chain |
725376 |
| 6.2.1.45 | more |
only full-length GSTHsUba1a is catalytically active |
674561 |
| 6.2.1.45 | more |
reduced survival rates of Saccharomyces cerevisiae strain MHY501 expressing the domains of ubiquitin-activating enzyme E1 under heat stress with or without inducer CuSO4, overview. Under antibiotic and heat stresses expression of the domains SCCH and UFD prove to be detrimental to cell survival |
-, 760825 |
| 6.2.1.45 | more |
removal of residues 330-404 of the C-terminal domain does not abrogate formation of the UBA5-ubiquitin fold modifier1 thioester intermediate, the UBA5 C-terminal domain is not required for adenylation or thioester transfer of ubiquitin fold modifier1 to the UBA5 catalytic cysteine |
709113 |
| 6.2.1.45 | more |
RNA antisense silencing of Giardia E1. Overexpression of E1 greatly increases the encystation rate |
-, 733043 |
| 6.2.1.45 | Q608R |
site-directed mutagenesis |
745863 |
| 6.2.1.45 | S621R |
site-directed mutagenesis |
745863 |
