EC Number   |
Protein Variants |
Reference |
|---|
   6.2.1.45 | D616R |
site-directed mutagenesis |
745863 |
| 6.2.1.45 | D623R |
site-directed mutagenesis |
745863 |
| 6.2.1.45 | E601R |
site-directed mutagenesis |
745863 |
| 6.2.1.45 | H614R |
site-directed mutagenesis |
745863 |
| 6.2.1.45 | K528A |
Km-value for ATP is 1.6fold higher than wild-type value, KM-value for ubiquitin is 2.9fold higher than wild-type value. kcat for ubiquitin adenylate formation is 400fold lower than wild-type value. kcat for ubiquitin carrier protein E2 transthiolation is 309fold lower than wild-type value |
674561 |
| 6.2.1.45 | K528A |
mutation within the MgATP2- binding site, results in dramatically impaired binding affinities for MgATP2-, a shift from ordered to random addition in co-substrate binding, and a significantly reduced rate of ternary complex formation that shifts the rate-limiting step to ubiquitin adenylate formation. Mutations does not affect the affinity of Ubc2b binding, however, differences in kcat values determined from ternary complex formation versus HsUbc2b transthiolation suggest that binding of the E2 enhances the rate of bound ubiquitin adenylate formation |
674561 |
| 6.2.1.45 | more |
a mutational reduction in Uba1 function (Uba1B2 mutation) reduces the efficacy of cell death, a complete loss of Uba1 function (Uba1A1 mutation) results in poor survival of mutant tissue and overgrowth of adjacent wild type tissue |
692413 |
| 6.2.1.45 | more |
construction of a mouse Aos1-Uba2 chimeric SUMO(small ubiquitin-related modifier)-E1 enzyme, mAU. The SUMO-E1 enzyme consists of two subunits, a heterodimer of activation of Smt3p 1 (Aos1) and ubiquitin activating enzyme 2 (Uba2), which resembles the N- and C-terminal halves of ubiquitin E1 (Uba1), the functional domains appear to be arranged in a fashion similar to Uba1. mAU has SUMO-E1 activity, indicating that mAU can be expressed in baculovirus-insect cells and represents a suitable source of SUMO-E1, enzymatic mechanism and structure of SUMO-E1, overview |
733442 |
| 6.2.1.45 | more |
enzyme silencing in HEK293 cells by lentiviral expression of shRNA. Generation of orthogonal pairs of xUB-xUBA6 and xUB-xUBA1, analysis of orthogonal interaction of xUB-xE1 in mammalian cells and identification of xUB-conjugated proteins. 697 potential Uba6 targets and 527 potential Uba1 targets with 258 overlaps are identified |
745863 |
| 6.2.1.45 | more |
genotyping-phenotyping |
735079 |
