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Results 1 - 2 of 2
EC Number Crystallization (Commentary) Reference
Show all pathways known for 6.2.1.25Display the word mapDisplay the reaction diagram Show all sequences 6.2.1.25purified recombinant His-tagged wild-type enzyme, hanging drop vapor diffusion method, mixing of 0.002 ml of 17.5 mg/ml protein in 20 mM Tris, pH 8.0, with or without 4 equiv carboxylic acid ligand, e.g. 2-fluorobenzoate, 2-methylbenzoate, 3-furoate, and thiophene-2-carboxylate, with 0.002 ml of reservoir solution containing 0.1 M Tris-HCl, pH 7.0, and 15% w/v PEG 3350, best crystals grew in a pH range from pH 6.5-7.5, X-ray diffraction structure determination and analysis 744341
Show all pathways known for 6.2.1.25Display the word mapDisplay the reaction diagram Show all sequences 6.2.1.25sitting-drop vapor diffusion method at 18 °C in 23% PEG 3350 K and 0.2 M sodium formate 675454
Results 1 - 2 of 2