EC Number   |
|---|
    5.4.2.12 | - |
| 5.4.2.12 | crystals of iPGAM complexed with 3-phosphoglycerate and Co2+, hanging-drop vapor diffusion, crystallization drops contain equal volumes of reservoir and enzyme solution, 5 mg/ml iPGAM, 1.5 mM 3-phosphoglycerate, 50 mM NaCl, 0.01 mM CoCl2 in 20 mM TEA buffer, pH 7.4, crystals diffract to 1.9 A |
| 5.4.2.12 | crystals of iPGM complexed with 3-phosphoglycerate and Mn2+ at 1.9 A resolution |
| 5.4.2.12 | crystals of wild-type and S62A mutant at 1.4 A resolution |
| 5.4.2.12 | hanging drop vapor diffusion method, using 0.2 M NaCl, 0.1 M Bis/Tris pH 6.3 and 25% (w/v) polyethylene glycol 3350 |
| 5.4.2.12 | iPGAM crystallized with 3-phosphoglycerate or 2-phosphoglycerate at high and low cobalt concentrations (4 mM and 0.01 mM), hanging drop vapor diffusion method, using 0.08 M ammonium acetate, 0.04 M trisodium citrate dihydrate pH 6.0, and 24% (w/v) PEG 4000 |
| 5.4.2.12 | methionyl and selenomethionyl phosphoglycerate mutase, vapor drop hanging diffusion, mixing equal volumes of protein solution, i.e. 50 mg/ml protein in 150 mM 3-phosphoglycerate, pH 7.4 and reservoir solution containing 2 M ammonium sulfate, 25 mM zinc acetate, 20 mM cesium chloride, 15 mM 2-mercaptoethanol, 3% polyethylene glycol 200 and 50 mM Tris-HCl, pH 7.4, crystals diffract to 2.5 A resolution |
| 5.4.2.12 | protein is composed of two structural and functiional domains, the phosphatase and the transferase. Comparison with the structurally similar domains of in Bacillus stearothermophilus |
| 5.4.2.12 | purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, mixing of 0.003 ml of 8 mg/ml protein solution containing 20 mM Tris-acetate-EDTA, pH 7.4, 50 mM NaCl, 001 mM CoCl2, with 0.003 ml of reservoir solution containing 0.05 M ammonium sulfate, 0.1 M Bis-Tris, pH 6.1, and 25% w/v PEG 3350, 18°C, X-ray diffraction structure determination and analysis at 2.3 A resolution |
| 5.4.2.12 | sitting drop vapor diffusion method, using either 0.1 M HEPES pH 7.5, 20% (w/v) polyethylene glycol 10000 or 0.2 M NaCl, 0.1 M bis-Tris pH 6.5, 25% (w/v) polyethylene glycol 3350, at 25°C |
