EC Number |
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5.1.3.7 | hanging drop vapor diffusion method, crystal structure of the enzyme (TMGalE) and the enzyme bound to UDP-glucose is determined at resolutions of 1.9 A and 2.0 A, respectively |
5.1.3.7 | hanging drop vapor diffusion method, crystal structure of the enzyme in complex with cofactor and either UDP-glucose or UDP-N-acetyl-D-galactosamine, determined at 2.5 and 2.1 A, crystals belong to the space group P222(1), with unit cell dimensions of a = 61 A, b = 96 A, and c = 140 A |
5.1.3.7 | microbatch under oil method, using 250 mM ammonium sulfate, 100 mM Bis-Tris pH 6.5, (25% w/v) PEG 3350 |
5.1.3.7 | purified recombinant mutant H305A enzyme, best by microbatch under oil set up and hanging drop vapor diffusion method, from a solution containing 25% PEG 3350, 0.2 M ammonium sulfate, cryoprotection by 15-20% glycerol, X-ray diffraction structure determination and analysis at 2.10 A resolution |
5.1.3.7 | purified recombinant unbound enzyme TMGalE, and TMGalE bound to UDP-Glc, X-ray diffraction structure determination and analysis at 1.9-2.0 A resolution |