EC Number   |
|---|
    5.1.3.3 | - |
| 5.1.3.3 | both apo-form and in complex with alpha,beta-D-galactose |
| 5.1.3.3 | both apoform and in complex with beta-D-galactose, comparison with enzyme from Lactococcus lactis and Caenorhabditis elegans |
| 5.1.3.3 | hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol 4000, 200 mM sodium acetate and 100 mM Tris-HCl pH 8.5 |
| 5.1.3.3 | hanging-drop vapour-diffusion method |
| 5.1.3.3 | in complex with D-glucose, D-fucose, D-quinovose, L-arabinose, or D-xylose |
| 5.1.3.3 | in complex with NAD+, UDP-glucose, and beta-D-galactose |
| 5.1.3.3 | mutants H96N, H170N, D243N, D243A, E304Q, E304A, complexed with either D-glucose or D-galactose |
| 5.1.3.3 | purified recombinant His-tagged enzyme, hanging-drop vapour-diffusion method, 0.003 ml of 10 mg/ml protein in 15-25% PEG 8000, 0.1 M Na citrate, pH 5.5, and 0.2-0.5 mM NaCl for monoclinic crystals, or in 1.5-2 M ammonium sulfate, 25 mM Mg formate, 0.1 M Na cacodylate, pH 5.5, for orthorhombic crystals, cocrystallization of YeaD with ligands glucose, galactose, mannose, fructose, fucose, xylose, lyxose, lactose, maltose, sucrose and glucose 6-phosphate, incubation at 4°C for 12 h in a 1:10 molar ratio, X-ray diffraction structure determination and analysis the orthorhombic form at 1.9 A resolution and in the monoclinic form at 2.5 A resolution |
