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EC Number Crystallization (Commentary)
Show all pathways known for 4.2.1.25Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.25hanging-drop vapour-diffusion method at 20°C. Crystals of RlArDHT that diffract to 2.4 A resolution are obtained using sodium formate as a precipitating agent. They belong to space group P21, with unit-cell parameters a = 106.07, b = 208.61, c = 147.09 A, beta = 90.43°
Show all pathways known for 4.2.1.25Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.25the active site contains a catalytically important [2Fe-2S] cluster and Mg2+ ion and is buried between two domains, and at the dimer interface. The active site Lys129 is carbamylated. Ser480 and Thr482 are essential residues for catalysis, and the S480A mutant structure shows an open conformation in which the active site is more accessible for the substrate. The alkoxide ion form of the Ser480 side chain may function as a base and the [2Fe-2S] cluster may function as a Lewis acid in the elimination reaction
Show all pathways known for 4.2.1.25Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.25to 2.4 A resolution, space group P21, with unit-cell parameters a = 106.07, b = 208.61, c = 147.09 A , beta = 90.43°
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