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EC Number Crystallization (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.88MdcD-MdcE subunits, sitting drop vapor diffusion method, using 0.1 M Bis-Tris (pH 5.8), 18% (w/v) PEG 3350, and 0.2 M ammonium acetate. Holoenzyme in complex with CoA and malonate, sitting drop vapor diffusion method, using 20% (w/v) PEG 3350 and 8% (w/v) tacsimate
Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.88the holoenzyme contains four subunits, having an acyl-carrier protein (MdcC subunit) with a distinct prosthetic group, as well as decarboxylase (MdcD-MdcE) and acyl-carrier protein transferase (MdcA) catalytic activities. Structure of the MdcD-MdcE complex at 1.86 A resolution, and overall strcuture of the heterotetramer at 2.2 A resolution
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