3.6.1.31 | native and selenomethionine-labeled, uncomplexed enzyme in two crystal forms, hanging-drop vapor diffusion method, 10 mg/ml protein in 20 mM HEPES, pH 7.5, mixing of protein solution and reservoir buffer containing 100 mM sodium cacodylate pH 6.5, 1.0 M sodium citrate, 0.1 M guanidinium hydrochloride in a 1:1 ratio, 4 days, X-ray structure determination and analysis at 1.25 A and 1.79 A resolution, respectively |
695322 |