3.5.99.10 | purified recombinant enzyme RidA in the apo form, as well as RidA complexed with pyruvate, sitting drop vapor diffusion method, crystallization from reservoir solution containing 18% PEG 3350, 0.1 M MES, pH 6.0, 25°C, 10 days, soaking of apo-crystals in 20 mM pyruvate for 1 hour, X-ray diffraction structure determination and analysis at 2.3 A resolution |