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EC Number Crystallization (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 3.5.99.10purified recombinant enzyme RidA in the apo form, as well as RidA complexed with pyruvate, sitting drop vapor diffusion method, crystallization from reservoir solution containing 18% PEG 3350, 0.1 M MES, pH 6.0, 25°C, 10 days, soaking of apo-crystals in 20 mM pyruvate for 1 hour, X-ray diffraction structure determination and analysis at 2.3 A resolution
Display the word mapDisplay the reaction diagram Show all sequences 3.5.99.10sitting drop vapor diffusion method, using 16% (w/v) PEG 10000, 0.1 M bis-Tris pH 5.3 and 0.1 M ammonium acetate
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