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EC Number Crystallization (Commentary)
Show all pathways known for 3.5.2.5Display the word mapDisplay the reaction diagram Show all sequences 3.5.2.5-
Show all pathways known for 3.5.2.5Display the word mapDisplay the reaction diagram Show all sequences 3.5.2.5purified recombinant His6-tagged enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml 14 mg/ml protein in 20 mM Tris-HCl, pH 8.0, with 0.001 ml of reservoir solution containing 20% w/v PEG 3350, and 0.1 M potassium thiocyanate, pH 6.5, equilibration against 0.5 ml reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 3.5 A resolution
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