EC Number   |
|---|
   3.5.1.46 | purified recombinant Hyb24 by sitting drop vapor diffusion from 0.1 M MES, pH 6.5, 2.02.2 M ammonium sulfate, 0.10.2 M lithium sulfate, at 10°C, 2 ml of sample mixed with 2 ml of reservoir solution, preparation of HgCl2 heavy atom derivatives, X-ray diffraction structure determination and analysis at 1.8 A resolution |
| 3.5.1.46 | purified recombinant Hyb24, sitting drop vapor diffusion, 38 mg/ml protein in 20 mM phosphate, pH 7.3, and 10% glycerol, 0.02 ml of protein solution is mixed with the same volume of reservoir solution and equilibrated against 0.8 ml of reservoir solution, 10°C, 48 h, preparation of methylmercuric chloride heavy atom derivatives, X-ray diffraction structure determination and analysis at 1.75-1.8 A resolution |
| 3.5.1.46 | purified wild-type and mutant (Hyb-24DNY-G187A112, Hyb-24DNY-S187A112, or Hyb-24DNY-A187A112) enzymes free or in complexes with 6-aminohexanoate, X-ray diffraction structure determination and analysis |
| 3.5.1.46 | sitting-drop vapor-diffusion method, three-dimensional structures of the complex of 6-aminohexanoate-dimer hydrolase and 6-aminohexanoate-linear dimer and the complex of S112A-mutant of 6-aminohexanoate-dimer hydrolase and 6-aminohexanoate-linear dimer |
