   3.4.24.87 | purified recombinant detagged ADAMTS13-DTCS, residues 287-685, an non-catalytic von Willebrand factor-exosite-containing ADAMTS13 fragment, sitting drop vapor diffusion method, mixing of 500 nl of protein solution and 500 nl reservoir solution containing 26% w/v PEG1500, 100 mM MES, pH 6.0, supplemented with 200 nl of 40% w/w pentaerythritol ethoxylate, equilibration for several days at 20°C. Os-derivative crystals by soaking native crystals in reservoir solution supplemented with 1 mM OsCl3 and 20% glycerol for several hours, X-ray diffraction structure determination and analysis at 2.6 A and 2.8 A resolution resulting in two crystal structures |
| 3.4.24.87 | recombinant His-tagged ADAMTS13 ancillary domains, residues 287-685, sitting-drop vapour-diffusion method, mixing of 100 nl protein solution, containing about 20 mg/ml protein, with an equal amount of reservoir solution, containing 26% w/v PEG 1500, 100 mM MES, pH 6.0, equilibration against 0.1 ml of reservoir solution, at 20°C, for 24 h, crystals are soaked in a solution containing 20% glycerol, 26% PEG 1500, 100 mM MES, pH 6.0, for cryoprotection, X-ray diffraction structure determination and analysis at 2.6-2.8 A resolution, labeling with heavy-atom derivatives |
