EC Number   |
|---|
   3.4.24.68 | - |
| 3.4.24.68 | crystals obtained with the hanging drop method |
| 3.4.24.68 | in complex with ganglioside GD1a, hanging drop vapor diffusion method, using 20% (w/v) polyethylene glycol 3350, 0.1xa0M Bis-Tris propane pH 6.5, 0.2xa0M potassium thiocyanate. In complex with ganglioside GM1a, sitting drop vapor diffusion method, using 20% (w/v) polyethylene glycol 6000, 0.1xa0M MES pH 6.0, 0.2xa0M sodium chloride |
| 3.4.24.68 | purified C-terminal receptor binding domain with bound ganglioside GT2 in presence and absence of lactose, 10 mg/ml protein in 20 mM Tris-HCl buffer, pH 7.9, containing 100 mM NaCl are mixed with the carbohydrate moiety of GT2 at 1:8 molar ratio, vapor diffusion hanging drop method, 0.002 ml of protein-ligand solution are mixed with 0.002 ml of well solution containing 100 mM bis(trispropane) buffer, pH 7.0, 25% polyethylene glycol 2000 and 300 mM ammonium sulfate, equilibration against 0.5 ml well solution at 19°C, X-ray diffraction structure determination and analysis at 2.0-2.1 A resolution, molecular replacement method |
| 3.4.24.68 | sitting drop vapor diffusion method. The structure provides insight into the active site, the importance of the nucleophilic water and the role of the zinc ion |
| 3.4.24.68 | tetanus neurotoxin C fragment, vapor-diffusion method, hanging drops and sitting drops, thick rod-shaped crystals, space group P2(1)2(1)2(1), unit cell dimensions a : 67.4 A, b : 79.7 A, c : 91.1 A |
