   3.4.22.46 | foot-and-mouth disease virus 3C protease mutant C95K/C142L/C163A complexed with a peptide substrates APAKQLLNFD and APAKELLNFD that spans P5-P5', 1:5.5 molar ratio of enzyme to peptide, 0.05 ml of of 3Cpro solution at 17 mg/ml protein in 100 mM HEPES, pH 7.0, 400 mM NaCl, 1 mM EDTA, 2 mM 2-mercaptoethanol, and 0.01% w/v sodium azide is mixed with 0.007 ml of 30 mM peptide dissolved in the same buffer, yielding a final protein concentration of 14.9 mg/ml, sitting-drop vapour diffusion, room temperature, mixing of 0.001 ml of protein complex solution with 0.003 ml reservoir composed of 40-43% PEG 400, 0.2 M LiSO4 and 0.1 M Tris, pH 8.0 X-ray diffraction structure determination and analysis |
709535 |
