3.4.22.29 | purified recombinant enzyme, hanging-drop vapour diffusion method, mixing of 200 nl of 10 mg/ml protein in 20 mM HEPES, pH 7.0, 50 mM NaCl, and 10% glycerol, with 200 nl of reservoir solution containing 0.2 M potassium chloride, 0.1 M magnesium acetate tetrahydrate, 0.05 M sodium cacodylate, and 10% PEG 8000, equilibration against 0.1 ml of reservoir solution, 16°C, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular replacement using the structure of 2Apro from coxsackievirus A16 (CVA16) as the search model |