3.2.1.75 | native enzyme or in complex with beta-glucosyl-1,6-deoxynojirimycin, hanging drop vapor diffusion method, using 0.2 M sodium bromide, 0.1 M Bis-Tris propane, pH 6.5, 20% w/v PEG 3350 or 0.2 M potassium thiocyanate 20% w/v PEG 3350, respectively |