3.2.1.180 | purified recombinant wild-type enzyme, sitting drop vapor diffusion method, mixing of 0..001 ml of 10 mg/ml protein in n 20 mM Tris-HCl, pH 7.5, 1 mM dithiothreitol, and 1 mM EDTA, with 0.001 ml of reservoir solution containing 25% PEG 6000, 0.1 M HEPES, pH 7.5, and 0.1 M lithium chloride, 20°C, 2 months, X-ray diffraction structure determination and analysis at 1.3 A resolution, molecular replacement |