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EC Number	Crystallization (Commentary)
3.2.1.149	purified recombinant alpha-mannosidase-treated enzyme, in complex with disaccharide amidine inhibitors, sitting drop vapor diffusion method, mixing of 0.0015 ml of 10 mg/ml protein in 10mM sodium acetate, with 0.0015 ml of reservoir solution containing 0.01% Triton X-100, 21% PEG 4000, 0.3 M ammonium acetate, and 0.1 M sodium citrate, pH 5.5, 20°C, X-ray diffraction structure determination and analysis at 1.8-1.9 A resolution, molecular replacement

EC Number

Crystallization (Commentary)

3.2.1.149

purified recombinant alpha-mannosidase-treated enzyme, in complex with disaccharide amidine inhibitors, sitting drop vapor diffusion method, mixing of 0.0015 ml of 10 mg/ml protein in 10mM sodium acetate, with 0.0015 ml of reservoir solution containing 0.01% Triton X-100, 21% PEG 4000, 0.3 M ammonium acetate, and 0.1 M sodium citrate, pH 5.5, 20°C, X-ray diffraction structure determination and analysis at 1.8-1.9 A resolution, molecular replacement

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Release 2023.1 (January 2023)