EC Number   |
|---|
   3.1.21.10 | - |
| 3.1.21.10 | apoenzyme, sitting drop vapor diffusion method, using 14% (w/v) PEG3350, 0.15 M NH4Cl. Mutant enzyme I112V/Q162K/E235Q/E239Q sitting drop vapor diffusion method, using 0.1M Bis-Tris, pH 6.3, 22% (w/v) PEG3350, 0.2 M Li2SO4. Mutant enzyme I112V/Q162K/E235Q/E239Q in complex with Holl8iday junctions, sitting drop vapor diffusion method, using 0.1 M sodium acetate trihydrate, pH 5.0, 8.5% (w/v) PEG3350, 2% Tacsimate, pH 4.0 or 0.1 M sodium acetate trihydrate, pH 5.6, 15% (w/v) 3-methyl-1,5-pentane diol, 2% (w/v) PEG4000, 2% (w/v) D-sorbitol |
| 3.1.21.10 | at 17°C, hanging drop method, in solution having a mushroom-like appearance, where the cap is formed by the main beta-sheet and flanking helices, structure of RecU superimposes best with PfHjc and archaeal HJR, the next best matches to RecU are a series of type II restriction endonucleases including EcoRV and Pvull |
| 3.1.21.10 | crystal structure of human HJ resolvase GEN1 complexed with DNA at 3.0 A resolution |
| 3.1.21.10 | crystal structure of Sulfolobus Hjc, determined by using multiple-wavelength anomalous dispersion, to a resolution of 2.15 A |
| 3.1.21.10 | hanging drop vapor diffusion at 7°C |
| 3.1.21.10 | hanging drop vapor diffusion method, using 35% (w/v) polyethylene glycol 3350, 0.2M Li2SO4 and 0.1M Tris-HCl (pH 8.5) |
| 3.1.21.10 | hanging-drop method at 20°C |
| 3.1.21.10 | hanging-drop method, 2.1 A resolution |
| 3.1.21.10 | hanging-drop method, 2.4 A resolution |
