EC Number |
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3.1.1.58 | alignment with PDB database: hits to tetratricopeptide repeat domains of human nucleoporin O-linked N-acetylglucosamine transferase, yeast mitochondrial outer membrane translocon protein Tom70p, and human peroxisomal targeting signal-1 receptor PEX5, putative domains: porin domain for export of poly-beta-1,6-N-acetyl-D-glucosamine, and periplasmic domain for protein-protein interactions |
3.1.1.58 | purified enzyme, hanging drop vapor diffusion method, mixing of 0.002 ml of 24-28 mg/ml protein in 25 mM Tris base, pH 8.0, 300 mM NaCl, and 10% glycerol with 0.002 ml reservoir solution containing 0.1 M Tris base, pH 8.0, 0.2 M NaCl, 30-34% PEG 3350, and equilibration against a 0.5 ml reservoir solution, 1-2 days, X-ray diffraction structure determination and analysis at 2.20 A resolution, energy-dispersive X-ray spectroscopy |
3.1.1.58 | purified recombinant enzyme, hanging drop vapour diffusion, protein in 50 mM HEPES-NaOH, pH 6.8, 200 mM NaCl is mixed with 25-30% w/v PEG 3350, 100 mM Tris-HCl, pH 7.5-8.0, X-ray diffraction structure determination and analysis at 1.90 A resolution, molecular replacement procedure, method overview. Construction of putative models of the C-terminal domain of the protein using a multitude of homology modelling algorithms, and test for the presence of signal in molecular replacement calculations, slow-cooling torsion-angle simulated annealing |