EC Number   |
Reference |
|---|
    2.8.3.5 | 14°C, hanging drop vapor diffusion, structure determination with X-ray crystallography |
672216 |
| 2.8.3.5 | hanging drop vapor diffusion method, using 20% (w/v) PEG 3350 and 100 mM Tris-HCl (pH8.5) |
724277 |
| 2.8.3.5 | hanging drop vapor diffusion method, using 75 mM Tris-HCl pH 8.0, 20% (w/v) PEG 3350 or PEG 4000 |
723826 |
| 2.8.3.5 | orthothrombic crystal form |
660636 |
| 2.8.3.5 | purified recombinant C28S and C28A mutants, hanging drop vapour diffusion method, enzyme in 0.125-0.5 M KCl, 0.5 mM benzamidine, 0.2 mM EDTA, 20 mM 2-mercaptoethanol, and 5 mM Tris-HCl, pH 8.0, the precipitant solution contains 14-22% polyethylene glycol 2000, 0.1 M Tris-HCl, pH 8.0, and 10-15% glycerol, X-ray diffraction structure determination and analysis at 2.0-2.05 A resolution |
672216 |
| 2.8.3.5 | purified recombinant ScoA and ScoB, sitting drop vapour diffusion method, 69 mg/ml protein in 20 mM Hepes, pH 8.0, 200 mM NaCl and 1 mM DTT, mixing with reservoir solution containing 100 mM Tris, pH 8.5, 200 mM MgCl2, and 20% PEG 8000, at 16°C, X-ray diffraction structure determination and analysis at 2.89 A resolution |
676985 |
| 2.8.3.5 | sitting drop vapor diffusion method, using 0.2 M lithium sulfate, 0.1 M bis-Tris pH 5.5, 25% (w/v) PEG 3350 |
737365 |
| 2.8.3.5 | sitting drop vapor diffusion method, using 0.20 M sodium chloride, 0.1 M Tris pH 9.0 and 25% (w/v) polyethylene glycol 3350 |
738807 |
