EC Number   |
|---|
    2.7.7.24 | co-crystallization with dTDP-glucose or dTMP and alpha-D-glucose 1-phosphate or deoxythymidine and alpha-D-glucose 1-phosphate, hanging-drop vapour-diffusion method in 1.7 M ammonium sulfate and 0.1 M sodium acetate, pH 5.3 |
| 2.7.7.24 | enzyme in complex with either dTTP or dTDP-alpha-D-glucose, hanging drop vapor diffusion method, using either 18-22% (w/v) poly(ethylene glycol) 8000 and 100 mM MOPS (pH 7.0) or 20% (w/v) poly(ethylene glycol) 3350, 200 mM KCl and 100 mM CHES (pH 9.0), respectively |
| 2.7.7.24 | homology modeling of both isoforms DnmL and RmbA |
| 2.7.7.24 | in complex with dTDP-alpha-D-glucose and diphosphate, sitting drop vapor diffusion method, using 0.2 M sodium sulfate, 0.1 M bis-Tris propane pH 8.5, 20% (w/v) PEG 3350 |
| 2.7.7.24 | sitting-drop vapour-diffusion method in polyethylene glycol 6000, 0.1 M sodium citrate, pH 4.6 and 0.5 M Li2SO4 as precipitant, crystallization as apoenzyme or co-crystallization with dTTP or thymidine and alpha-D-glucose 1-phosphate or dTDP-D-glucose or dTDP-L-rhamnose |
| 2.7.7.24 | sitting-drop vapour-diffusion method in polyethylene glycol 6000, 0.1 M sodium citrate, pH 4.6 and 0.5 M Li2SO4 as precipitant, space group: P1, the asymmetric unit contains eight monomers in the form of two RmlA tetramers with a solvent content of 51% |
| 2.7.7.24 | sitting-drop vapour-diffusion technique with PEG 6000 and lithium sulfate as precipitant. Several diffraction data sets of single frozen crystals are collected to a resolution of 1.66 A. Crystals belong to space group P1, with unit-cell parameters a = 71.5 A, b = 73.1 A, c = 134.7 A, alpha = 89.9°, beta = 80.9°, gamma = 81° |
