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Results 1 - 10 of 14 > >>
EC Number Crystallization (Commentary) Reference
Show all pathways known for 2.7.1.30Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.30- 641286, 641294, 641295, 641299, 641300, 641317, 641327, 702293, 705779
Show all pathways known for 2.7.1.30Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.30crystals are grown at 20°C by the sitting-drop vapour diffusion method. Native X-ray diffraction data are collected to 2.4 A resolution using synchrotron radiation at station BL44XU of SPring-8. The crystal belongs to the rhombohedral space group R3, with unit-cell parameters a = b = 217.48, c = 66.48 A. The protein is also cocrystallized with substrates and diffraction data are collected to 2.7 A resolution 684168
Show all pathways known for 2.7.1.30Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.30crystals are obtained by the hanging-drop technique from a solution containing 29% polyethylene glycol 400, 0.1 M sodium acetate pH 4.5, 0.1 M calcium acetate and 10% glycerol. The crystals can grow in the presence of 33% PEG 400, which allows to mount the crystals and directyl flash-cool them. Repeated flash-annealing causes a significant decrease in the averaged mosaicity along with an increase in the overall peak counts of reflections and an enhanced signal-to-noise ratio. Individual reflection-profile analysis reveales a mostly dual domain structure, showing the minimization of one domain as a result of flash-annealing. 677308
Show all pathways known for 2.7.1.30Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.30crystals of native and mutant enzyme with bound glycerol, hanging drop vapor diffusion method 661122
Show all pathways known for 2.7.1.30Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.30in complex with glycerol, ADP and the allosteric effector enzyme IIAGlc 661122
Show all pathways known for 2.7.1.30Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.30in complex with glycerol, in presence and absence of fructose 1,6-diphosphate, mechanism 641326
Show all pathways known for 2.7.1.30Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.30in complex with substrates, sitting drop vapor diffusion method, using M HEPES pH 7.5, 11% (v/v) hexane-1,6-diol, 25% (w/v) PEG400 739102
Show all pathways known for 2.7.1.30Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.30of wild type and mutant A65T, both in complex with glycerol and ADP, and of mutant I474D, in complex with IIAGlc 641320
Show all pathways known for 2.7.1.30Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.30purified recombinant His6-tagged enzyme in complex with pNPP, sitting drop method, mixing of 0.001 ml of 5 mg/mL TbgGK in 10 mM MgSO4, 20 mM NaCl, 0.001% glycerol and 10 mM MOPS, pH 6.8, with 0.001 ml of 11% 1,6-hexanediol, 25% PEG 400, and 0.1 M HEPES, pH 7.5, at 20°C, crystals of the TbgGK-pNPP complex are obtained by soaking TbgGK crystals in a cryoprotectant solution containing 5.5% 1,6-hexanediol, 40% PEG 400, and 0.1 M HEPES, pH 7.5, that is supplemented with 20 mM pNPP, at 20°C, for 6-14 h, X-ray diffraction structure determination and analysis, molecular replacement method using the protein structure of the TbgGK-ADP complex (PDB ID 3WXL) as a search model 760620
Show all pathways known for 2.7.1.30Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.30purified recombinant wild-type enzyme in complex with glycerol and AMPPCP, and enzyme mutant K271E free or in complex with glycerol, sitting drop vapor diffusion method, 4-5 mg/ml protein in 5 mM Tris-HCl, pH 7.5, with or without 5 mM glycerol, and 5 mM AMPPCP, is mixed with 0.1 M HEPES, pH 8.0, containing 15% w/v PEG 1000 and 200 mM calcium acetate, 20°C, X-ray diffraction structure determination and analysis at 2.3-3.1 A resolution, molecular replacement using the structure of Tk-GK (PDB ID 2ZF5) as template, modelling 761561
Results 1 - 10 of 14 > >>