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Results 1 - 4 of 4
EC Number Crystallization (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.82crystal structures of YgjG at 2.3 and 2.1 A resolutions for the free and putrescine-bound enzymes, respectively. YgjG forms a dimer that adopts a class III pyridoxal 5'-phosphate-dependent aminotransferase fold. Structures of YgjG and other class III aminotransferases are similar. YgjG has an additional N-terminal helical structure that partially contributes to a dimeric interaction with the other subunit via a helix-helix interaction. The YgjG substrate-binding site entrance size and charge distribution are smaller and more hydrophobic than other class III aminotransferases 739501
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.82hanging-drop vapour-diffusion method, PEG 3350, pH 7.5 723860
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.82purified recombinant enzyme in apoform, sitting drop vapour diffusion method, best from 0.2 M MgCl2, 0.1 M Bis-Tris, pH 6.5, and 25% PEG 3350, at 20°C, X-ray diffraction structure determination analysis at 2.1 A resolution, molecular replacement method, structure modelling using the structure of aspartate aminotransferase from Pseudomonas sp. (PDB ID 5TI8) as the search model 759219
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.82purified recombinant His-tagged enzyme, sitting drop vapour diffusion metod, mixing of 150 nl of 10.5 mg/ml protein in 25 mM Tris chloride, 137 mM NaCl, and 3 mM KCl, pH 8.0, with 150 nl of reservoir solution containing 0.066 M calcium acetate, 18% w/v PEG MME 5000, 0.1 M Tris chloride, pH 7.6, and equilibration against 0.5 ml of reservoir solution, at 20°C, method optimization, X-ray diffraction structure determination analysis at 2.07 A resolution, molecular replacement method, structure modelling using the structure of a Chromobacterium violaceum omega transaminase monomer (PDB ID 4a6t) as the search model 758596
Results 1 - 4 of 4