EC Number |
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2.6.1.57 | ARO9 by hanging drop vapour diffusion method, X-ray diffraction structure determination and analysis at 2.6 A resolution |
2.6.1.57 | crystal structure of unliganded pdAroAT, pdAroAT in a complex with maleate and 3-phenylpropionate at 2.33 A, 2.5 A and 2.3 A resolution |
2.6.1.57 | hanging drop vapor diffusion, an equi-volume of 3 M 1,6-hexanediol solution at pH 7.5, 100 mM HEPES buffer, containing 10 mM MgCl2 is added to a protein solution containing 1.6% AeATPh and 0.02 mM pyridoxal 5'-phosphate, a droplet of the solution is equilibrated with 1 ml of 3 M 1,6-hexane-di-ol solution, crystals are grown at room temperature for 1 week, crystal structure of the native enzyme 2.1 a resolution, heavy atom derivatives diffract to 3.0 A resolution |
2.6.1.57 | hanging-drop vapor diffusion method at 20°C using 3 M 1,6-hexanediol, 100 mM HEPES (pH 7.5), and 10 mM MgCl2 as the precipitant solution. The crystal structure of the ligand-free enzyme is analyzed at a resolution of 1.8 A |
2.6.1.57 | purified recombinant enzyme, hanging drop vapor diffusion method, mixing of 0.001 ml of 18.0 mg/ml protein in 25 mM Tris-HCl, pH 7.0, with 0.001 ml of reservoir solution containing 0.2 M potassium chloride, pH 7.0, and 20% w/v PEG 3550, and equilibration against 0.02 ml reservoir, X-ray diffraction structrue determination and analysis at 2.2 A resolution, molecular replacement method using the crystal structure of AroAT from Pyrococcus horikoshii strain OT3 (PDB ID 1dju) as initial search model, modeling |
2.6.1.57 | tertiary structures of pdAroAT complexed with nine kind of inhibitors: 3-indolebutyric acid, 4-phenylbutyric acid, 5-phenylvaleric acid, 4-(2-thienyl)butyric acid, cyclohexanepropionic acid, 4-amminohydrocinnamic acid, 3-(p-tolyl)-propionic acid and 3-(3,4-dimethoxyphenyl)propionic acid, crystals of the maleate complex of pdAroAT are made by the micro-seeding method using 24% poly(ethylene)glycol 4000, 200 mM sodium maleate, pH 5.7 and 5 mM MgCl2 as precipitating buffer, cocrystallized inhibitor maleate is replaced by soaking the crystals in 24% poly(ethylene)glycol 4000, 100 mM sodium citrate, pH 5.7 containing 100 mM inhibitor for 10 h |