EC Number   |
|---|
    2.5.1.86 | crystallization using sparse matrix screens and vapor diffusion, structures of Rv2361c in the apo form, with isopentyl diphosphate bound and with a substrate analogue, citronellyl diphosphate |
| 2.5.1.86 | enzyme in complex with the substrate analogues geranyl S-thiodiphosphate and isopentenyl S-thiodiphosphate, sitting drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 10% (v/v) glycerol, 25% (w/v) PEG 400, 7% (w/v) PEG 3000 |
| 2.5.1.86 | in complex with BPH-640, hanging drop vapor diffusion method, using |
| 2.5.1.86 | purified recombinant enzyme in complex with inhibitor BPH-640, X-ray diffraction structure determination and analysis at 1.8-1.9 A resolution |
| 2.5.1.86 | sitting-drop vapour-diffusion method at 25°C. A 1.55 A resolution structure of the enzyme in complex with the substrate analogues geranyl S-thiodiphosphate and isopentenyl S-thiodiphosphate bound to their respective sites in one subunit shows the active-site configuration and the magnesium-coordinated geometry for catalysis |
