EC Number |
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2.4.2.14 | crystal structure at 3.0 A |
2.4.2.14 | crystal structure in the absence of ligands at 2.0 A, and with bound AMP at 2.5 A |
2.4.2.14 | crystal structure of 6-diazo-5-oxonorleucine inactivated enzyme at 2.3 A resolution |
2.4.2.14 | crystals of the ternary enzyme-ADP-GMP complex are grown in glass melting point capillaries by the microbath method, 20 mg/ml enzyme are incubated with 1 mM ADP, 1 mM GMP, and 5 mM MgCl2 and mixed with an equal volume of 24% polyethylene glycol 8000, 200 mM KCl, 50 mM N-(2-hydroxyethyl)piperazine-N'-3-propanesulfonic acid, pH 7.9, brown crystals emerge after 6-12 weeks |
2.4.2.14 | sitting drop vapor diffusion method, using 0.1 M sodium citrate tribasic pH 5.6, 1.5 M ammonium phosphate monobasic, and 0.1 M citric acid pH 3.4 |
2.4.2.14 | when mapped onto the structure from Bacillus subtilis GPRAT (PDB: 1AO0), the site of mutation R264K lies within 10 A of the glutaminase site |