EC Number   |
|---|
   2.4.1.248 | crystal structure of the core fragment from Ser39 to Met738, devoid of its N-terminal signal peptide and C-terminal nonconserved regions. The structural model contains one catalytic (beta/alpha)8-barrel domain and three beta-domains. Domain N with an immunoglobulin-like beta-sandwich fold is attached to the N-terminus. Domain C with a Greek key beta-sandwich fold is located at the C-terminus, and a carbohydrate-binding module family 35 beta-jellyroll domain B is inserted between the 7thbeta-strand and the 7th alpha-helix of the catalytic domain A. The structures of the inactive catalytic nucleophile mutant enzyme complexed with isomaltohexaose, isomaltoheptaose, isomaltooctaose, and cycloisomaltooctaose reveal that the ligands bind in the catalytic cleft and the sugar-binding site of CBM35 |
| 2.4.1.248 | crystals of CITase bearing an N-terminal His6 tag, resolution of 2.3 A, belong to space group P3121, containing a single molecule in the asymmetric unit.. Crystals of CITase bearing a C-terminal His6 tag, resolution of 1.9 A, belong to space group P212121, containing two molecules in the asymmetric unit |
| 2.4.1.248 | sitting-drop vapour diffusion method at 20°C, crystal structure of the enzyme is determined in its apo- and cycloisomaltooligosaccharides (CI-7) complex forms |
