EC Number   |
|---|
   2.3.1.B43 | crystal structure of Sirt5 in complex with a thioacetyl peptide is obtained, The corresponding acetyl peptide could not be crystallized with Sirt5 |
| 2.3.1.B43 | crystal structure of Sirt5 in complex with fluor-de-Lys peptide and resveratrol and crystal structure of Sirt5 in complex with fluor-de-Lys peptide and piceatannol |
| 2.3.1.B43 | crystal structures of a binary complex of SIRT5 with an H3K9 succinyl peptide and a binary complex of SIRT5 with a bicyclic intermediate obtained by incubating SIRT5-H3K9 thiosuccinyl peptide co-crystals with NAD+ |
| 2.3.1.B43 | crystals of native and selenomethionine-derivatized cobB are grown at room temperature using the hanging-drop, vapor-diffusion method. The crystal structure of the Escherichia coli cobB core domain (residues 40274) in complex with an 11-residue peptide containing residues 1219 of histone H4 and acetylated at lysine 16 is determined by a combination of Zn2+ and Se multiwavelength anomalous diffraction to 1.96 A resolution |
| 2.3.1.B43 | hanging drop vapor diffusion method at 18°C. X-ray crystal structures of the ternary complex of SIRT5 bound to a peptide substrate and carba-NAD+ (an unreactive NAD+ analogue) |
| 2.3.1.B43 | hanging drop vapor diffusion method at 4°C, crystal structure of wild-type enzyme, mutant enzyme S24A, mutant enzyme H80N, mutant enzyme F159A, and triple Sir2 mutant (D102G/F159A/R170A) |
| 2.3.1.B43 | hanging drop vapor-diffusion method at 20°C. Crystal structures of SIRT5 in complex with ADP-ribose, and crystal structures of SIRT5 bound to suramin |
| 2.3.1.B43 | purified recombinant detagged enzyme in complex with peptide inhibitors 2 and 15, sitting drop vapor diffusion method, mixing of 10 mg/ml protein with 1 mM inhibitor, crystallization in 0.002 ml of reservoir solution containing 0.1 M HEPES, pH 7.5, and 20% PEG 3350, 20°C, 2-4 days, X-ray diffraction structure determination and analysis at 2.4 A resolution, molecular replacement with search model PDB 4UTV and modeling |
| 2.3.1.B43 | purified recombinant enzyme SIRT5 in complex with succinylated histones, crystals of Sirt5-H4K122su and Sirt5-H2AK95su complexes are obtained from 0.1 M sodium cacodylate, pH 5.5, and 25% w/v PEG 4000 at 22°C. Sirt5-H4K91su complex is crystallized from 0.1 M Bis-Tris, pH 5.5, and 25% w/v PEG 3350, and Sirt5-H2BK120su complex is grown in 0.2 M sodium acetate trihydrate, 0.1 M sodium cacodylate trihydrate, pH 6.5, and 30% w/v PEG 8000, X-ray diffraction structure determination and analysis at 1.70-2.30 A resolution, molecular replacement using the structures PDB ID 3RIY as a searching model |
| 2.3.1.B43 | vapor diffusion at 20°C, crystal structure of the enzyme bound to KGLGKGGA(N6-succinyl)KRHRKW |
