1.3.8.9 | purified recombinant cVLCAD, hanging-drop vapour-diffusion method, 0.002 ml of 12 mg/ml protein in 20 mM Tris-HCl pH 8.0, 150 mM NaCl are mixed with 0.002 ml of reservoir solution containing 100 mM Tris-HCl, pH 8.0, 150 mM NaCl, 200 mM magnesium formate and 13% PEG 3350, 16°C, 3 days, X-ray diffraction structure determination and analysis at 1.8-2.6 A resolution, molecular replacement |